Article Dans Une Revue BMC Research Notes Année : 2025

Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus

Résumé

Objectives: This study investigated the efficacy of daptomycin against adherent Staphylococcus aureus (S. aureus), a common colonizer of medical devices that leads to severe infections. For the first time, we evaluated the bactericidal effects of daptomycin on S. aureus immediately after adhesion, mimicking early-stage contamination of biomaterials. Time-kill curve assay and confocal laser scanning microscopy (CLSM) were used to analyze the process dynamics. In addition, atomic force microscopy (AFM) and scanning electron microscopy (SEM) were employed to elucidate daptomycin-induced structural changes in the bacterial cell wall. Results description: Daptomycin, at clinically relevant concentrations, rapidly eradicated adherent bacteria in the exponential growth phase, demonstrating an efficiency comparable to its action against planktonic cells. Prolonged exposure to the antibiotic caused marked alterations in the bacterial cell wall, including surface roughening and perforation, as revealed by multimodal imaging. However, daptomycin effectiveness diminished as biofilm formation progressed, underscoring the need for further exploration of optimized clinical strategies.
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hal-04940845 , version 1 (11-02-2025)

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Alexis Canette, Rym Boudjemaa, Julien Deschamps, Karine Steenkeste, Christian Marlière, et al.. Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus. BMC Research Notes, 2025, 18 (1), pp.54. ⟨10.1186/s13104-025-07130-5⟩. ⟨hal-04940845⟩
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